Abstract: The effects of PBL teaching mode in the experimental teaching of Pharmacology in application-oriented colleges were discussed. Taking 30 students in Class 2 of Grade 2008 in traditional Chinese pharmacology speciality of Anhui Science and Technology University as experiment group, traditional LBL teaching mode was used in the teaching. The satisfaction degree of student in experiment group on PBL teaching mode, experimental operation ability, experimental report＇s writing and results analysis were investigated by questionnaire. The results showed that introducing PBL teaching mode into the experimental teaching of Pharmacology could significantly enhance the teaching effects, and this mode was suitable for being popularized in experimental teaching of Pharmacology in application-oriented colleges.