Abstract: Examination is an important link in teaching process and also a significant way to evaluate the student learning effect assessment. Animal physiology is one of the important professional basic courses in agriculture and forestry universities. The Animal Physiology course was used as an example, and examination was put throughout the teaching curriculum by reforming content and form of examination. The results showed that, scientific and reasonable examination could urge the students to take the use of time to study effectively, then reduced the flunk rate, and also had a certain promotion in improving the teaching quality.