Abstract: The virtual simulation experiment is based on the virtual simulation technology which is comprehensively integrated by various disciples and can simulate the real experimental environment. Via the introduction of virtual simulation experiment to the experimental teaching of Animal Biochemistry, the experimental cost is reduced, the experimental content is expanded, and the contradictions between teaching requirement and experimental resources deficiency are effectively solved. The statistically analysis on performance of two grades of undergraduates demonstrates that the application of virtual simulation experimental teaching mode improves the teaching quality and cultivates the undergraduates＇ ability to find, analyze and solve problems.