Abstract: [Objective]To assess the influence of students′ participation in Chinese Traditional Medicine Chemistry experiment preparation on the teaching effect of this experiment course. [Methods]The detailed evaluation indexes for teaching effect evaluation are set up, and the teaching effect of the different methods applied in students from three grades are compared.[Results]The better teaching effect is observed in the students with participation in experiment preparation compared to those without participation. [Conclusion]The participation in experiment preparation significantly improve the teaching effect of Chinese Traditional Medicine Chemistry experiment.