Abstract: In an attempt to further improve the teaching effect of experimental course of Animal Biochemistry, to enhance the undergraduates′ practical ability and the familiarity on experimental course, and to deepen their understanding of the theory course, the blended teaching method was preliminary applied in experimental course of Animal Biochemistry. The results showed that the average scores of the experimental group (undergraduates taught with the blended teaching method, 91.25±2.48) were improved by 1.78% compared to the control group (those taught with the traditional teaching method, 89.65±2.19), and the average scores of the undergraduates in experimental group after receiving the blended teaching method (91.35±2.48) were improved by 4.58% compared to before receiving the blended teaching method (87.35±3.35). In addition, during the whole process of experimental course teaching, higher participation and initiative was observed in the undergraduates taught with the blended teaching method, suggesting the teaching effect of experimental course of Animal Biochemistry was obviously improved.