Abstract: Experimental animals play important roles in the experimental teaching of Animal Function. In order to implement the rational utilization of experimental animals in experiment teaching, firstly, the teaching content in different courses is needed to be integrated and optimized and the comprehensive experiment projects are needed to be designed to reduce experimental animal usage;secondly, the teaching means and methods in experimental teaching are expected to be improved by replacing the using of experimental animals with computer aided experiment method, meanwhile, it was necessary to make the undergraduates familiar to the process of the experiments by combining computer aided experiment method with traditional experimental methods, so as to reduce the unnecessary loss of experimental animals; thirdly, 3R animal welfare alternatives should be promoted, the related knowledge on experimental animal welfare and ethics are supposed to be propagated for the undergraduates, so as to guide them to cherish the opportunity to participate experimental courses and to strictly control the experimental cost.