Abstract: Taking the independent-sample t test by using Excel and SPSS as an example, the analysis process of inferencing the two samples data in randomly designed experiment was interpreted to investigate the statistical results between two treatments. In terms of two independent-sample t test, the data obtained with Excel were completer and more accurate but were complicated; the analysis process with SPSS was relatively simple, but the obtained data were less accurate than Excel. It was indicated that the choice of Excel and SPSS in independent-sample t test was expected to be made according to the actual situation.