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Effects of the growth and development of potatoes and soil nutrients in potato fields based on soaking seeds with different microbial agents
LIU Yana, CHEN Wenjin, FAN Yafang, WEI Hong, ZHAO Yinglin, XUE Huiling, ZHANG Jing, KONG Qingquan, HE Xiaoyong, ZHANG Ziyi
Journal of Northern Agriculture    2024, 52 (2): 26-36.   DOI: 10.12190/j.issn.2096-1197.2024.02.03
Abstract2956)      PDF(pc) (1396KB)(295)       Save
【Objective】To explore the effects of different microbial agents on potato growth and development and soil nutrients in potato fields,so as to provide a reference for green and sustainable potato planting in Inner Mongolia.【Methods】Potato V7 was used as the test material,by setting distilled water(CK),applying Bacillus sp. 188 bacterial agent(T1), Pseudomonas sp. BP16 bacterial agent(T2),and Streptomyces rochei D74 bacterial agent(T3) four treatments to soak the seeds for 30 min. Potato yield,tuber starch content,dry matter mass of each organ,nutrient accumulation per plant,leaf chlorophyll content,nitrate reductase activity,soil ammonium nitrogen,nitrate nitrogen,available phosphorus and available potassium content were measured at different growth stages after potato planting.【Results】After 110 days of potato planting,compared with CK,the yield of potato in T2 and T3 treatments was significantly increased by 7.14% and 9.16%( P<0.05),the starch content of tubers was significantly increased by 11.84% and 16.38%( P<0.05),the dry matter mass of potato roots,stems,leaves,and tubers were significantly increased by 26.87%,16.92%,11.77% and 13.59% by T3 treatment( P<0.05),the accumulation of nitrogen,phosphorus and potassium in plants was significantly increased by 28.86%,32.78% and 30.81% by T3 treatment( P<0.05). After 70 days of potato planting,the chlorophyll content in potato leaves treated with T3 treatment was the highest [4.29 mg/(g·FW)],and the nitrate reductase activity in the leaves was significantly increased by 15.29% compared to CK( P<0.05). After 50 days of potato planting,the soil nitrate nitrogen content in T3 treatment reached its maximum value(42.32 mg/kg),the available phosphorus content significantly increased by 26.17% compared to CK( P<0.05),and the available potassium content in T1 and T3 treatments significantly increased by 24.95% and 21.65% compared to CK( P<0.05),respectively.After 70 days of planting,the soil ammonium nitrogen content in T2 treatment significantly increased by 47.03% compared to CK( P<0.05).【Conclusion】Soaking seeds with Streptomyces rochei D74 microbial can increase potato yield,tuber starch content,dry matter mass of each organ,plant nitrogen,phosphorus and potassium accumulation,at agent tuber formation stage,the leaf chlorophyll content at maturation stage,soil nitrate nitrogen,available potassium,available potassium content at early growth stage,soil ammonium nitrogen at agent tuber formation stage. It can be used as a suitable microbial agent for potato cultivation in Inner Mongolia Autonomous Region.
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Evaluation index system of maize varieties suitable for mechanical grain harvest in Inner Mongolia
SHI Haibo, LIANG Hongwei, ZHANG Jing, YANG Jingyuan, HAN Ping′an, LIU Zhixiong, FENG Yong, SU Erhu, ZHANG Laihou, ZHAO Ruixia
Journal of Northern Agriculture    2024, 52 (2): 1-14.   DOI: 10.12190/j.issn.2096-1197.2024.02.01
Abstract2841)      PDF(pc) (1269KB)(270)       Save
【Objective】The evaluation index system of maize varieties suitable for mechanical grain harvest in Inner Mongolia was established to provide the basis for the evaluation,selection and timely harvest of maize varieties suitable for mechanical grain harvest in Inner Mongolia,and to point out the direction for variety breeding.【Methods】The representative varieties of six maturity groups from extremely early maturity to late maturity were used as experimental materials to study the mechanical grain harvest traits simultaneously in six ecological regions of Inner Mongolia,at the physiological maturity,10,20 and 30 d after physiological maturity,the grain breakage rate,grain fragmentation rate,impurity rate,grain moisture content and plant lodging rate were measured;The varieties with suitable ecological regions conditions and excellent mechanical grain harvest traits were selected for the identification and selection of evaluation index of varieties suitable for mechanical grain harvest.【Results】Grain breakage rate,grain fragmentation rate and impurity rate have an extremely significant positive correlation with grain moisture content( P<0.01). The grain moisture content at harvest stage has an extremely significant negative correlation with the time and accumulated temperature from emergence to harvest,from silking to harvest,and from maturity to harvest( P<0.01),and the correlation increased in turn.The grain dehydration rate has an extremely significant positive correlation with the basic moisture content and daily average temperature( P<0.01). The regression equation of grain dehydration rate ( y) with basic moisture content ( x 1) and daily average temperature ( x 2) was y=-0.523 318+0.022 732 x 1+0.025 422 x 2, R 2=0.419 2, P<0.01. The total yield loss rate and ear drop rate have an extremely significant positive correlation with lodging rate of plants at harvest stage( P<0.01),but grain drop rate was not significantly correlated with lodging rate of plants at harvest stage( P>0.05). There was no significant correlation between plant lodging rate and plant height,ear height,ratio of ear height to plant height,stem diameter,ratio of stem diameter to plant height( P>0.05). The primary and secondary index for the evaluation of maize varieties suitable for mechanical grain harvest were established,covering 3 levels and 55 index.【Conclusion】The evaluation index system of maize varieties suitable for mechanical grain harvest in Inner Mongolia was established.It takes into account the commonness and regional individuality of each ecological region,and the index is quantified and easy to operate. The provision of the “grain fragmentation rate” index points out the invisible loss in the process of mazie mechanical grain harvest,which makes the evaluation of yield loss more comprehensive.
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Effects of increased application of organic fertilizer and microbial fertilizer on agronomic traits and yield of soybean
ZHAO Xiaoyu, CHEN Guangping, SU Erhu, WANG Xuejiao, LIU Kunyu, GAO Yuanli, E Lili, TANG Cunxi, HONG Di, LI Qiang
Journal of Northern Agriculture    2024, 52 (2): 37-45.   DOI: 10.12190/j.issn.2096-1197.2024.02.04
Abstract2416)      PDF(pc) (1348KB)(140)       Save
【Objective】In order to investigate the effects of increased application of organic fertilizer and microbial fertilizer on soybean growth and development in Hulun Buir City,Inner Mongolia and the economic benefits,and provided a reference for soybean water and fertilizer management in this area.【Methods】The experiment will be carried out in the Morin Dawa Daur Autonomous Banner of Hulun Buir City from 2022 to 2023. Mengdou No. 48 was used as the test material,and three fertilization treatments were set up,conventional fertilization(CK),increased application organic fertilizer(OF),and increased application of microbial fertilizer(MF),respectively. The experiment analyzed soybean growth traits,leaf SPAD value,dry matter accumulation and yield factor indexes,and calculated economic benefits,and calculated synergism and efficiency increase.【Results】The plant height,number of main stem nodes and effective branching number of soybean treated with OM were significantly higher than those treated with CK( P<0.05),and the bottom pod height of soybean treated with OM and MF was significantly lower than that treated with CK( P<0.05). The changes of dry matter accumulation and leaf SPAD value of soybean increased gradually with the progression of growth stage,and the performance was OM>MF>CK.Dry matter accumulation at podding stage,drumming stage and maturity stage was significantly higher under OM treatment than CK( P<0.05). The leaf SPAD value of OM treatment was significantly higher than CK at flowering stage,podding stage and druming stage( P<0.05). In the past two years,the number of pod per plant,grain weight per plant,100-grain weight and effective plant number of soybean in OM and MF treatment were significantly higher than those in CK( P<0.05),and there was no significant difference between OM and MF treatment( P>0.05). Soybean yield has a extremely significant positive correlation with number of pod per plant,number of seeds per plant,grain weight per plant and effective plant number( P<0.01). Soybean yield was significantly positively correlated with whole growth period,plant height,number of main stem nodes,dry matter accumulation,leaf SPAD value and 100-grain weight( P<0.05). Soybean yield was significantly negatively correlated with bottom pod height( P<0.05). In the past two years,the soybean yield under OM treatment increased by 625.70 kg/hm 2 and 599.60 kg/hm 2 compared with CK,and the benefit increased by 21.20% and 19.30%,respectively.In addition,MF treatment increased production by 375.80 kg/hm 2 and 237.10 kg/hm 2 compared with CK,and the benefit increased by 10.40% and 3.80%,respectively.【Conclusion】In Hulun Buir City,Inner Mongolia,the best fertilizer mode for increasing soybean yield and efficiency was to apply organic fertilizer on the basis of conventional fertilizer.
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Evaluation and identification index screening of shade-tolerant soybean varieties(lines)suitable for maize-soybean intercropping in northern Jiangsu
LIU Shuhua, ZHANG Lijie, ZHOU Lingling, YU Xiang, TIAN Fufa, MENG Jiali, WU Shaojun, SHEN Hong, YANG Nianfu, ZHANG Yanliu
Journal of Northern Agriculture    2024, 52 (2): 15-25.   DOI: 10.12190/j.issn.2096-1197.2024.02.02
Abstract2257)      PDF(pc) (1118KB)(38)       Save
【Objective】Screening shade-tolerant soybean varieties(lines) suitable for maize-soybean intercropping planting mode in northern Jiangsu,and constructing an accurate and reasonable soybean shade tolerance evaluation system.【Methods】The natural shade environment created by maize-soybean intercropping in northern Jiangsu was used as the treatment,and the clear seed was used as the control. 12 agronomic traits such as plant height,pod height,and branch number of 24 soybean varieties(lines) were measured at maturity stage. Principal component analysis,membership function method,and multiple linear stepwise regression analysis were used to construct shade tolerance coefficients and evaluate the comprehensive shade tolerance of each soybean variety(lines) under intercropping mode.【Results】Index of shade tolerance coefficient of 12 agronomic traits were transformed into 4 independent comprehensive indexes,representing 74.771% of the original data information. The results of comprehensive shade tolerance evaluation value( D) and cluster analysis showed that 24 soybean varieties(lines) could be divided into 3 categories according to their adaptability under shade stress,including strong shade-tolerant(six varieties/lines),moderate shade-tolerant(nine varieties/lines)and weak shade-tolerant(nine varieties/lines). The regression equation of shade tolerance was constructed by multiple linear stepwise regression analysis: D=-1.158+0.062 X 2+0.249 X 5+0.201 X 7+1.002 X 10+0.665 X 11R 2=0.972 2),and the fitting accuracy was more than 89.14%. Five shade tolerance identification indexes of pod height,pod number per plant,seed number per plant,100-seed weight and yield were selected.【Conclusion】According to the shade tolerance ability,the 24 soybean varieties(lines) were divided into three categories:strong shade-tolerant,moderate shade-tolerant and weak shade tolerant. Under the maize-soybean intercropping model in northern Jiangsu,five traits,including pod height,number of pods per plant,number of grains per plant,100 grain weight,and yield,can be selected for comprehensive evaluation of soybean shade tolerance. Under the maize-soybean intercropping mode in northern Jiangsu,five traits,including pod height,pod number per plant,seed number per plant,100 seed weight,and yield,could be selected to comprehensively evaluate the shade tolerance ability of soybean.
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Genome-wide association analysis for maize stem nutritional quality traits and candidate gene selection
WANG Bangtai, YANG Meili, GUO Hua, WANG Jing, WANG Zhihong, LU Hongwei, CHENG Jianmei, QIN Guiwen, CHEN Jiafa
Journal of Northern Agriculture    2023, 51 (5): 1-22.   DOI: 10.12190/j.issn.2096-1197.2023.05.01
Abstract2072)   HTML6)    PDF(pc) (9949KB)(135)       Save

【Objective】Conduct genetic analysis of the main nutritional quality traits of maize stems at the molecular level,to provide theoretical basis for genetic improvement of the main nutritional quality traits of maize stems.【Methods】The study used 381 different types of maize inbred lines as associated populations and planted randomly in the experimental field of the Hebi Academy of Agricultural Sciences in Henan Province in 2016 and 2017. The nutritional quality traits acid washing fiber(ADF),neutral washing fiber(NDF),and digestibility(IVDMD) were investigated during the heading,filling,and maturity stages. Using the Maize SNP50 gene chip,461 053 high-quality SNPs were obtained for genome-wide association analysis.【Results】Using the generalized linear model(GLM) analysis,a total of 392 significantly correlated stem nutritional quality traits SNPs(P<1.08×10-7) were detected on 10 chromosomes of maize during different growth stages of the two years,with phenotypic variation interpretation rates of 9.69% -17.34%. 97 SNPs were found to be independently associated more than twice,resulting in a total of 77 candidate genes associated with stem nutritional quality traits. Using the mixed linear model(MLM)analysis,a total of 257 significantly correlated SNPs were detected over the two years(P<2.17×10-6),with phenotypic variation interpretation rates of 9.82% -20.20%. 17 SNPs were found to be independently associated more than twice,resulting in a total of 82 candidate genes. Among them,10 overlap with GLM model candidate genes. A total of 159 candidate association genes were identified by GLM and MLM,among which 43 candidate genes were detected twice or more. Zm00001d021261 was found to be associated with ADF,NDF,and IVDMD in different years 31 times at multiple loci. This gene encoded ATP binding protein and chloroplast sensor kinase,had the highest expression in leaves at maturity stage. Zm00001d021255 was found to be associated with stem nutritional quality traits 10 times at two loci. This gene encoded cysteine rich receptor like protein kinase 25,which was only expressed in developmental internodes and female panicles,can be further studied and validated.【Conclusion】Using GLM and MLM models could quickly and effectively associate significant loci related to maize stem nutrient quality. HC-SNP could be screened through repetitive significant loci,and then associated gene enrichment regions could be excavated.

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Review of progress in chickpea (Cicer arietinum) research
CHEN Wenjin;KONG Qingquan;ZHAO Cunhu;HE Xiaoyong;TIAN Xiaoyan;XI Xianmei(Plant Protection Institute,Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences,Hohhot 010031,China)
Journal of Northern Agriculture    2019, 47 (2): 119-123.   DOI: 10.3969/j.issn.2096-1197.2019.02.21
Abstract2055)      PDF(pc) (980KB)(3621)       Save
Chickpeas have high nutritional value and are the third most important bean crop in the world.Chickpea distribution range,nutritional value and medicinal value are reviewed in this paper.Previous research undertaken on chickpeas is introduced,current problems and the development of countermeasures are described,and the future development of chickpeas in China is considered.The review provides a reference for research chickpeas.
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Effects of continuous application of soil conditioner on the growth,development and yield components of maize in acidic soil in the southeastern region of Liaoning
ZHANG Lili, XUE Bingdong, FAN Ye, MO Jiaojiao, ZHAO Xinyu, YANG Hailong, FU Jun, JIANG Ying, QI Hua, WANG Pu
Journal of Northern Agriculture    2023, 51 (4): 38-47.   DOI: 10.12190/j.issn.2096-1197.2023.04.05
Abstract1893)   HTML4)    PDF(pc) (1080KB)(130)       Save

【Objective】To explore the effects of continuous application of soil conditioner quicklime and organic fertilizer on the growth,development,and yield components of maize in acidic soil in the southeastern region of Liaoning.【Methods】From 2019 to 2021 field experiments were conducted in Fengcheng City,Liaoning Province. Four treatments were set up including no application of soil conditioner(CK),quicklime(CaO),organic fertilizer(Organic),and organic fertilizer+quicklime(Organic+CaO). The effects of continuous application of soil conditioner on maize plant traits,dry matter accumulation,yield components,and other indicators were analyzed.【Results】From 2019 to 2021,the maize yield of CaO treatment were all higher than CK. The yield of Organic and Organic+CaO treatments were higher than CK in the first two years and were lower than the CK in the third year of continuous application. The yield increase of CaO treatment were in the range of 2.12% to 19.74%. The yield increase rates of Organic+CaO treatment were 15.74% and 7.89% in the first two years,respectively. But there was no yield increase effect in the third year of application. The year,soil conditioner,and their interaction had extremely significant effect(P<0.01) on maize yield. The yield of CaO and Organic+CaO treatments were significantly higher(P<0.05) than CK and the Organic treatments. The ear number of CaO treatment was significantly higher(P<0.05) than CK. CaO and Organic+CaO treatments had significantly higher(P<0.05) kernel number per ear and kernel number per hectare than that of CK and Organic treatment. 1 000-kernel weight in Organic+CaO treatment was significantly higher(P<0.05) than all other treatments. Yield was significantly positively correlated(P<0.05) with kernel number per ear (R2=0.614) and kernel number per hectare (R2=0.746). Continuous application of soil conditioner could improve plant height,ear height,and the ratio of ear height to plant height of maize. And the effect on ear height was more than the effect on plant height. Leaf area index (LAI) increased rapidly in early growth stages and decreased slowly in later growth stages. The effects were more obvious for Organic+CaO and Organic treatments. Applying soil conditioner promoted the dry matter accumulation and post floral dry matter accumulation of all the growth stages. The effect of Organic+CaO treatment was better than that of Organic and CaO treatments.【Conclusion】Continuous application of CaO and Organic+CaO on acidic soil promoted the maize growth and development. However,from the yield prospective,when using Organic+CaO as soil conditioner in the southeastern region of Liaoning,continuous application for more than two years should be avoided.

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Genetic diversity analysis of phenotypic traits in 99 barley germplasm resources
GUO Chengyu, YI Fengyan, FANG Yongyu, SHI Zhidan, LIU Fang, ZHAO Heping, DING Haijun
Journal of Northern Agriculture    2023, 51 (4): 1-10.   DOI: 10.12190/j.issn.2096-1197.2023.04.01
Abstract1858)   HTML9)    PDF(pc) (1197KB)(145)       Save

【Objective】Identify the phenotypic traits of different types of barley germplasm resources,to provide basis for breeding practice.【Methods】The diversity of 8 qualitative traits including seedling habits,leaf posture,plant type,ridge type,awn type,awniness,naked/cover and grain color of 99 barley germplasm resources were analyzed by descriptive statistics.Using descriptive statistics,correlation analysis,cluster analysis,and principal component analysis,10 quantitative traits including plant height,growth period,tiller number,main spike length,main spike grain number,main spike grain weight,spike number per plant,grain number per plant,grain weight per plant and 1 000-grain weight were analyzed.【Results】 There were abundant variations in the 8 qualitative traits. The variation range of 10 quantitative traits was 9.08%-43.16%,among which the variation coefficient of main spike grain number was the highest,and the variation coefficient of growth period was the lowest. Correlation analysis showed that there were significant(P<0.05) or extremely significant(P<0.01)correlations among multiple traits of the 99 barley germplasm. The germplasm population was divided into 4 categories by cluster analysis. The 15 accessions in theⅠcategory had the characteristics of high stem,long main spike and long growth period. The 56 accessions in the Ⅱ category had the characteristics of low stalk,high spike number per plant,high tiller number and long main spike. The 26 accessions in theⅢcategory had the characteristics of high main spike grain weight,high main spike grain number and low 1 000-grain weight. There were 2 accessions in the Ⅳ category,characterized by high grain number per plant,high grain weight per plant,low 1 000-grain weight. Principal component analysis showed that the cumulative contribution rate of the three principal components reached 72.39%.【Conclusion】 There were abundant genetic variations in 18 phenotypic traits of 99 barley germplasm resources,which could be divided into 4 categories. Breeding selection could be based on group characteristics.

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Research progress of maize dwarf genes and their regulatory mechanisms
TANG Lan, WU Yuanqi
Journal of Northern Agriculture    2023, 51 (5): 23-30.   DOI: 10.12190/j.issn.2096-1197.2023.05.02
Abstract1692)   HTML37)    PDF(pc) (853KB)(436)       Save

Plant height is an important indicator of the ideal plant type of maize and closely related to yield. It affects plant photosynthetic utilization,lodging resistance,harvest index,etc. By altering cell division and elongation,phytohormones change the length and quantity of maize internodes,modifying maize plant height to achieve the effect of dwarfing crops. This paper reviewed the research progress in recent years on the mapping and cloning of QTL/genes that control maize plant height and the formation of dwarf mutants under the regulation of hormones(GA3,IAA,BR),in order to provide references for the production and application of maize breeding.

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Evaluation of drought tolerance and salt tolerance of 16 maize varieties at germination stag
QIU Pengcheng, MIAO Yongmao, DU Yongchun, CHANG Guoyou, WU Yunxia
Journal of Northern Agriculture    2021, 49 (2): 11-16.   DOI: 10.12190/j.issn.2096-1197.2021.02.02
Abstract1676)      PDF(pc) (501KB)(102)       Save
【Objective】The drought tolerance and salt tolerance of 16 maize varieties at germination stage were evaluated.【Methods】Five treatments including 15% PEG-6000 drought stress,20% PEG-6000 drought stress,220 mmol/L NaCl salt stress,300 mmol/L NaCl salt stress and control(water)were set up. The drought tolerance and salt tolerance of 16 maize varieties were evaluated by weighted membership function value method. The GGE map was used to distinguish the tolerance of varieties. At the same time,the similarity and discrimination ability of different stress treatments in variety evaluation were analyzed.【Results】The weighted membership function value method evaluation results showed that,Yidan 131 and Zhenjin 308 were drought tolerant and Yidan 81 was salt tolerant;the GGE map was used to distinguish different stress treatments,the results showed that 15% PEG-6000,20% PEG-6000 and 300 mmol/L NaCl were positively correlated. The comprehensive evaluation of drought tolerance and salt tolerance of 16 maize varieties showed drought tolerance:Yidan 131,Yidan 81 and Zhenjin 308,and salt tolerance:Yidan 81,Yidan 507 and Shengfeng silage No.2.【Conclusion】Yidan 131 was a drought tolerant maize variety,and Yidan 81 was a drought and salt tolerant maize variety.
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Study on agrobacterium mediated transfer of CP4 -EPSPS gene into maize inbred line B73
HAN Ping′an, CHANG Yue, TANG Kuan′gang, LI Xiaodong, WANG Liwei, LIANG Yahui, YANG Jing, SHI Haibo, WU Xinrong
Journal of Northern Agriculture    2023, 51 (1): 31-37.   DOI: 10.12190/j.issn.2096-1197.2023.01.04
Abstract1612)      PDF(pc) (1131KB)(162)       Save
【Objective】Establish a maize genetic transformation system to cultivate maize lines with herbicide tolerance and address the issue of weed damage in maize.【Methods】Herbicide tolerance gene( CP4 -EPSPS)was transformed into immature embryos of maize inbred line B73 by agrobacterium mediated transformation. The transgenic plants were identified by PCR and qRT-PCR. Low copy transgenic events were screened by ddPCR,followed by phosphinothricin tolerance test.【Results】12 of the 181 acquired tolerance lines tested positive. The exogenous herbicide tolerance gene( CP4 -EPSPS)of the transgenic plants expressed normally at the transcriptional level. Five low copy lines were selected from the transgenic plants. All the transgenic plants were phosphinothricin tolerant in the tolerance test. T 1 seeds from the 5 transgenic low copy lines were harvested.【Conclusion】Established a maize genetic transformation system mediated by Agrobacterium transformation using B73.
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Research of VNTR molecular markers application in the cytoplasm type identification of sugarbeet
ZHANG Hui,WANG Liang,FU Zengjuan,LI Xiaodong,ZHAO Shangmin,E Yuanyuan,ZHENG Wenzhe,ZHANG Ziqiang,ZHANG Bizhou,ZHANG Huizhong
Journal of Northern Agriculture    2022, 50 (1): 1-10.   DOI: 10.12190/j.issn.2096-1197.2022.01.01
Abstract1606)      PDF(pc) (786KB)(123)       Save
【Objective】Sugarbeet paired maintainer lines and male sterile lines′ cytoplasmic types were determined in order to provide theoretical basis and technical advice for breeding sugarbeet maintainer lines and male sterile lines.【Methods】40 sugarbeet test materials were identified using the generated VNTR specific primers TR1, TR2, TR3,and TR4, and the cytoplasmic types were separated according to the particular bands, which was combined with the research of fertility features in the field.【Results】Among the 21 materials to be selected for sterility type,the materials conforming to be Owen type were N9849-17-1,N9865-103-C1, N9857-5-1-TH1-400, MS321-C27-1-80, MS331-N70, MS343-80, MS117-3-6-4-2, MS301-500, MS351-80, MS327-70-80, MS333-70-80, MS335-N70, 2068B-2, MS151-1-16-301-400, MS317-1-8-301, MS313-506-600 and MS320-7-605-1-84. MS329-N70 was cytoplasm type N1 and MS323-503-600 was N2. Among the 19 fertile materials to be selected, 960767-201TH-1, 960764-1-11-1-1-400, OT322-C7-70-80, OT332-N70, OT352-80, OT328-70-80, BS301-13-9, OT352-80-1, OT342-80 and OT152-1-6-301-400 were found to be in line with the cytoplasm type N1, and OT302-500 was found to be in line with the cytoplasm type N2. 960766-1077-c1, OT344-80, OT118-4-5-4-4 and OT324-501-600 were Owen type. Six materials with inconsistent molecular identification results were MS351-80-1, MS341-80, OT152-1-1-1-C301, OT330-N70, OT334-70-80, and OT336-N70. The results of field investigation revealed that 960766-1077-c1, OT344-80, OT118-4-5-4-4 and OT324-501-600 were semi-fertile with Owen cytoplasmic types. MS329-N70 and MS323-503-600 were semi-sterile type Ⅱ and were not Owen cytoplasmic type.【Conclusion】The VNTR specific primers developed could be utilized to distinguish the cytoplasmic types of sugarbeet maintainer lines and male sterile lines. The results were consistent with the results of the field fertility feature identification.
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Analysis on photosynthetic characteristics,dry matter accumulation,and yield of different spring maize varieties in dryland
ZHAO Li, FAN Mingyuan, XIE Guangming, LU Jingying, ZHANG Qi, SUN Xiaojing, GUO Hongxia, DENG Yan, WANG Chuangyun
Journal of Northern Agriculture    2023, 51 (4): 11-19.   DOI: 10.12190/j.issn.2096-1197.2023.04.02
Abstract1473)   HTML3)    PDF(pc) (1132KB)(331)       Save

【Objective】To screen varieties of dryland spring maize suitable for planting in Shanxi Province.【Methods】From 2020 to 2021,four spring maize varieties with good growth and high yield were selected as experimental materials and tested at two test sites of Chaoyang Town,Shouyang County and Dongyang Town,Yuci District in Jinzhong City. Using single factor randomized block design,the agronomic traits,photosynthetic characteristics,and dry matter accumulation of spring maize varieties were compared and the correlation analysis on yield and yield components were performed.【Results】At the test site in Chaoyang Town,Shouyang County,Dafeng 30 and Dika 159 had excellent agronomic traits,strong photosynthetic characteristics,high dry matter accumulation in leaves and panicles,and low dry matter accumulation in stems. The average yield increased by 3.59% and 1.70% compared to Taiyuan No. 9(CK),respectively. At the test site in Dongyang Town,Yuci District,the photosynthetic characteristics and dry matter accumulation of Dafeng 30 were significantly higher than those of Jindan 86 in the early growth stage,while there was no significant difference between the two in the later growth stage. Compared with Jinboshi 806(CK),the average yields of Dafeng 30 and Jindan 86 increased by 4.13% and 3.69%,respectively. The yield of spring maize was negatively correlated with bald tip length,but positively correlated with ear length,number of grains per ear,100-grain weight,and yield per plant.【Conclusion】Dafeng 30,Dika 159,and Jindan 86 were suitable for large-scale demonstration and promotion planting in the dryland spring maize planting areas of Shanxi.

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The impact of Sino-US trade friction on China′s international trade of agricultural products
ZHAO Jiping, ZHANG Jingxia, WU Yunxia
Journal of Northern Agriculture    2021, 49 (3): 110-115.   DOI: 10.12190/j.issn.2096-1197.2021.03.15
Abstract1451)      PDF(pc) (629KB)(742)       Save
【Objective】 Analyze the international trade situation of China′s agricultural products before and after the Sino-US trade friction and the impact of Sino-US trade friction on the international trade of China′s agricultural products,so as to provide references for the long-term stable development of China′s agriculture.【Methods】 Based on the data of China′s agricultural trade volume with the United States,China′s agricultural trade volume,and China′s import volume and transaction volume of major agricultural products from the United States published by the Ministry of Commerce,statistical analysis and literature research methods were used to analyze the situation and structure of international trade import and export of China′s agricultural products.【Results】 Sino-US trade friction had a huge impact on China′s agricultural international trade. On the one hand,China′s imports of major agricultural products in the United States have dropped from 32 855 600 tons of soybeans in 2017 to 16 943 600 tons in 2019,and wheat imports from 1 549 100 tons in 2017 decreased to 361 400 tons in 2018;China′s import transaction volume with the United States dropped from 31.741 billion US dollars in 2017 to 20.523 billion US dollars in 2019. The Sino-US trade friction has posed a certain threat to China′s food security and agricultural market stability;on the other hand,Sino-US trade frictions have prompted China to adjust its foreign trade structure and accelerate the diversified development of agricultural trade.【Conclusion】 By improving the import and export structure of international agricultural products trade,reducing its trade dependence on the United States,developing the EU and Southeast Asian markets,actively exploring new international trade markets and adopting a diversified market structure,China can improve the competitiveness of its own agricultural products while ensuring food security.
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Study on the spatial-temporal characteristics and driving factors of agricultural carbon emissions in Xinjiang
HUANG Xinhui, WANG Zhiqiang, OUYANG Qiwen, HUANG Xin, KANG Wenqin
Journal of Northern Agriculture    2024, 52 (1): 112-124.   DOI: 10.12190/j.issn.2096-1197.2024.01.13
Abstract1370)      PDF(pc) (1309KB)(122)       Save
【Objective】Study the spatial-temporal characteristics and driving factors of agricultural carbon emissions in Xinjiang,to provide references for promoting green development of agriculture and rural areas and achieving the“dual carbon”goals in the region.【Methods】Using 19 types of carbon sources from plantation,livestock and agricultural energy end-consumption as raw data,the agricultural carbon emissions and carbon emission intensities of Xinjiang from 2007 to 2021 were measured. Using 15 types of carbon sources from plantation and livestock as raw data,the carbon emissions and carbon emission intensities of fourteen prefectures(cities) in Xinjiang in 2010,2015,and 2020 were measured. The driving factors were analyzed by the LMDI(logarithmic mean divisia index) model. The grey prediction model was used to predict the trend of agricultural carbon emissions development in Xinjiang from 2022 to 2030.【Results】During 2007 to 2021,Xinjiang agricultural carbon emissions had the overall trend of“steady-increasing-decreasing-increasing”,while carbon emission intensities had the overall trend of “decreasing-increasing-decreasing”. In 2010,2015 and 2020,Ili Kazak Autonomous Prefecture had the highest total carbon emissions,Karamay had the lowest total carbon emissions,Kizilsu Kirgiz Autonomous Prefecture had the highest carbon emission intensities. Bayingol Mongolian Autonomous Prefecture had the lowest carbon emission intensities in 2010. Turpan had the lowest carbon emission intensities in 2015 and 2020. The impacts of driving factors on agricultural carbon emissions were:agricultural economic level effect > agricultural structure effect > agricultural population scale effect > agricultural production efficiency. From 2022 to 2030,the agricultural carbon emissions in Xinjiang were predicted to have an increasing trend while the intensities have a decreasing trend.【Conclusion】From 2007 to 2021,the total agricultural carbon emissions in Xinjiang showed an overall upward trend,while the agricultural carbon emission intensity showed an overall downward trend. Agricultural economic level effect promoted the increase of agricultural carbon emissions,while agricultural structure effect,agricultural population scale effect,and agricultural production efficiency restrained the increase of agricultural carbon emissions. It was predicted that from 2022 to 2030,the agricultural carbon emissions in Xinjiang will increase year by year,while the agricultural carbon emission intensities will decrease year by year. Xinjiang agricultural carbon reduction and fixation has enormous potential.
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A review of the development of maize root system and its configuration research
LIANG Yinlong, CHEN Liyi, WU Hanyan, YANG Yuxuan, WANG Xiaoyu, CHENG Yujia, ZHANG Yu
Journal of Northern Agriculture    2022, 50 (1): 59-65.   DOI: 10.12190/j.issn.2096-1197.2022.01.08
Abstract1366)      PDF(pc) (774KB)(701)       Save
One of the most important organs in plants is the root system. To maximize crop output potential,cultivating a healthy root system is critical. The growth,development,and configuration of maize root system have been studied extensively by researchers in China and overseas. This paper summarized the research results of maize root system characteristics,important factors affecting root system growth,root system configuration research methods,and main factors affecting root system configuration,based on the research progress of maize root system and its configuration,in order to provide a reference for future maize root system research.
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Screening and comprehensive evaluation of soybean varieties suitable for planting in the north foot of Yinshan Mountains
SUN Ying, WANG Fengwu, DAI Guixiang, ZHENG Chengzhong, MEI Xue, ZHANG Zizhen, WANG Qianjun, XU Zhenpeng, HUANG Wenjuan, YE Lu
Journal of Northern Agriculture    2023, 51 (3): 29-38.   DOI: 10.12190/j.issn.2096-1197.2023.03.04
Abstract1332)      PDF(pc) (798KB)(37)       Save
【Objective】To screen soybean varieties with high yield,superior quality and strong resistance suitable for planting in the north foot of Yinshan Mountains.【Methods】Variety comparison experiment was conducted on 20 soybean varieties provided by the Institute of Hulun Buir Agricultural Science to analyze the yield and quality traits. Cluster analysis and principal component analysis were used to comprehensively evaluate different soybean varieties.【Results】Cluster analysis divided the 20 varieties into three categories. The first category(Mengdou 50,Dengke No. 5,Mengdou 49,Dengke No. 1,Mengdou 58,Mengdou 912,Mengdou 57,Mengdou 42,Mengdou 1137,Mengdou 43,and Mengdou 160) comprehensively displayed the characteristics of high yield and high fat content,that was,high yield and superior quality. The second category(Mengdou 53,Mengdou 54,Mengdou 343,Mengdou 46,Mengdou 48,Mengdou 15,Neidou No. 4) had low yield and moderate quality. The third category(Dongnong 690) had high yield and low fat content. Principal component analysis showed that the yield factor and grain factor had contribution rates of 45.298% and 41.496%,respectively. The effective pods per plant,total seeds per plant,yield per plant,plot yield,fat content,and 100-seed weight all had high contribution.【Conclusion】Based on the results of principal component comprehensive evaluation ranking and cluster analysis,combined with comprehensive factors such as variety growth period and resistance,eight soybean varieties with good comprehensive performance were selected,including Mengdou 50,Mengdou 43,Mengdou 1137,Dengke No. 5,Mengdou 42,Mengdou 58,Mengdou 49,and Dengke No. 1. These soybean varieties were suitable for demonstration and promotion planting in the north foot of Yinshan Mountains.
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Analysis of the main virus diseases of facility tomato and pepper in Inner Mongolia
WANG Yanchun, AO Ni, CHEN Qi, XU Jia, YANG Chunxi, SONG Peiling, Gaowa, WANG Xiuzhi
Journal of Northern Agriculture    2023, 51 (1): 61-71.   DOI: 10.12190/j.issn.2096-1197.2023.01.07
Abstract1318)      PDF(pc) (991KB)(41)       Save
【Objective】To detect virus diseases of tomato and pepper in the primary protected vegetable production areas in Inner Mongolia,in order to identify the current dominant viral species,distribution,and infection status,as well as to assess the severity and prevalence of several significant virus diseases.【Methods】 From 2021 to 2022,tomato and pepper suspected virus disease samples were collected from the main protected vegetable production areas in Inner Mongolia including Chifeng City,Hohhot City and Baotou City. RT-PCR was used to identify the pathogens.【Results】A total of 327 virus infected samples were collected and 15 viruses were detected,including 12 in Chifeng City,8 in Hohhot City,and 7 in Baotou City.
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Effects of deep ploughing straw returning on root morphology and yield of maize in saline-alkali soil
ZHANG Hao, GAO Julin, YU Xiaofang, MA Daling, HU Shuping
Journal of Northern Agriculture    2023, 51 (1): 1-15.   DOI: 10.12190/j.issn.2096-1197.2023.01.01
Abstract1315)      PDF(pc) (1490KB)(109)       Save
【Objective】To elucidate the impacts of tillage methods on the development of saline-alkali farmland and maize growth by examining the growth and development of maize roots under deep ploughing straw returning.【Methods】From 2021 to 2022,two tillage methods,shallow rotation no returning(CK)and deep ploughing straw returning(DPR),were set up on mild,moderate and severe saline-alkali land. The local main maize varieties DK159 and JSH257 were used as experimental materials to analyze the effects of different treatments on soil physical and chemical properties,maize root growth status and yield.【Results】Compared with CK,the bulk density in DPR soil decreased,water content increased,alkaline hydrolytic nitrogen,total N,available P,available K and organic matter content increased significantly( P<0.05). The soil total salt content and pH value decreased by 5.48%-48.98% and 1.25%-13.71%,respectively. The root length,root surface area,average root diameter and root volume of DPR maize significantly increased( P<0.05). The root indexes improved most in the mild saline-alkali soil,with increases of 62.13%,62.99%,26.46% and 69.67%,respectively. In comparison with the CK,the yield of maize increased by 14.08%-33.61% with the highest increase in moderate saline-alkali soil.【Conclusion】Deep ploughing straw returning effectively improved soil physical and chemical properties,reduced soil total salt content and pH value. With the decrease of total salt content,maize root length and root volume increased significantly,increasing root surface area,improving the ability of maize plants to utilize soil nutrients,and ultimately increasing yield.
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Analysis of deoxynivalenol(DON) accumulation in wheat kernels and its correlation with test weight
HE Xianfang, DONG Xu, ZHAO Li, DU Binbin, LU Mao′ang, DENG Cheng, WANG Jianlai
Journal of Northern Agriculture    2022, 50 (5): 1-9.   DOI: 10.12190/j.issn.2096-1197.2022.05.01
Abstract1314)   HTML29)    PDF(pc) (947KB)(190)       Save

【Objective】To explore the accumulation of deoxynivalenol(DON) in the kernels of different wheat varieties under natural disease conditions and the effect on Fuasrium diseased kernel rate(FDK) and test weight,so as to provide reference for breeding wheat varieties resistant to Fuasrium.【Methods】The content of DON,3A-DON(3-acetyldeoxynivalenol) and 15A-DON(15-acetyldeoxynivalenol) in the kernels of 65 wheat varieties from different sources were tested by liquid chromatography-tandem mass spectrometry(LC-MS/MS) to analyze the difference in Fuasrium diseased kernel rate(FDK) and test weight (TW) under different DON content.【Results】The FDK of the 65 wheat varieties ranged from 0.20% to 7.80%. The content of DON was 0.11 to 4.26 mg/kg. The test weight was 703.00 to 791.70 g/L. With correlation coefficients of 0.514,0.549,0.529 and 0.521,respectively,FDK was significantly positively correlated with the contents of DON,3A-DON,15A-DON and DON-Total(P<0.01),and was significantly negatively correlated with test weight(P<0.05),with correlation coefficients of -0.254. With correlation coefficients of 0.999,0.911 and 0.899,respectively,the kernel contents of DON,3A-DON,15A-DON had significant positive correlation(P<0.01) with DON-Total,and significant negative correlation with test weight(P<0.01),with correlation coefficients of -0.502,-0.504 and -0.481,respectively. In terms of the source of the varieties,the kernel accumulation of DON was lowest in Jiangsu(1.51 mg/kg),followed by Anhui(2.06 mg/kg),Henan(2.75 mg/kg) and Shandong(2.98 mg/kg). The content of 3A-DON was lowest in Jiangsu(0.047 mg/kg),followed by Anhui(0.075 mg/kg),Shandong(0.111 mg/kg) and Henan(0.113 mg/kg). The content of 15A-DON was lowest in Jiangsu(0.059 mg/kg),followed by Anhui(0.090 mg/kg),Shandong(0.132 mg/kg)and Henan(0.141 mg/kg). The content of DON-Total was lowest in Jiangsu(1.61 mg/kg),followed by Anhui(2.23 mg/kg),Henan(3.00 mg/kg)and Shandong(3.23 mg/kg). The number and proportion of varieties with DON no more than 2 mg/kg in wheat kernels were as follows:Anhui(18,64.29%)>Jiangsu(13,61.90%)>Shandong(2,22.22%)>Henan(1,14.29%). The average test weight was highest in Jiangsu(758.57 g/L),followed by Anhui(751.90 g/L),Henan(739.71 g/L) and Shandong(735.27 g/L). In terms of the characteristics of different varieties,eight wheat varieties with DON no more than 2 mg/kg and test weight no less than 770.00 g/L were selected. Among these,there were seven semi winter varieties including Huaimai 40,Huaimai 44,Nongmai 152,Quanmai 725,Womai No. 9,Xunong 029 and Huaimai 45,one spring variety,Guohong No. 6.【Conclusion】The kernel FDK,DON contents and test weight of the 65 wheat varieties were quite different. With the increase of FDK,the kernel accumulation of 3A-DON,15A-DON and DON-Total increased gradually,and the average test weight decreased. Huaimai 40,Huaimai 44,Nongmai 152,Quanmai 725,Womai No.9,Xunong 029,Huaimai 45,and Guohong No. 6 had better resistance to Fuasrium disease.

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Effects of pre-seedling supplementary irrigation on the growth and photosynthetic characteristics of millet in the dry farming area of the Loess Plateau in Inner Mongolia
WU Yao, GAO Riping, CAI Linlin, HUANG Zhefan, CHEN Xiao, MEN Jingyu, ZHAO Peiyi, PAN Zhihua
Journal of Northern Agriculture    2023, 51 (5): 51-61.   DOI: 10.12190/j.issn.2096-1197.2023.05.05
Abstract1275)   HTML2)    PDF(pc) (1447KB)(11)       Save

【Objective】To screen excellent millet varieties suitable for planting in the dry farming area of the Loess Plateau in Inner Mongolia.【Methods】Using 8 millet varieties as research objects and split plot experiment design,two treatments of pre-seedling supplementary irrigation and water deficit were set up. The growth characteristics and photosynthetic characteristics of millet varieties were compared and analyzed by principal component analysis.【Results】Pre-seedling supplemental irrigation significantly affected the growth and photosynthetic characteristics of millet varieties. Compared with water deficit treatment,Jinmiao K1 had the highest (6.56%) seedling emergence rate,followed by Zhangzagu No. 13(4.44%);Jinmiao K1 and Zhangzagu No. 13 had better plant height performance,at 30.5 cm and 30.3 cm,respectively,increased by 15.21% and 6.27% and were significantly different from other varieties(P<0.05);Zhangzagu No. 13 had better stem diameter and dry matter accumulation at 5.45 mm and 0.598 g,respectively,increased by 12.60% and 5.65%,and were significantly different from other varieties(P<0.05),followed by Jinmiao K1,which increased by 16.56% and 4.98%,respectively. Under the water deficit treatment,the leaf area index of Zhangzagu No. 13 and Jinmiao K1 were less affected by drought at 0.94 and 0.83,respectively;the relative chlorophyll content of Zhangzagu No. 13 was the highest at 51.18. The stomatal conductance of Zhangzagu No. 13 and Jinmiao K1 was better under both treatments,significantly higher than that of other varieties(P<0.05),4.45% and 4.36% higher under pre-seedling supplemental irrigation treatment than water deficit treatment,respectively. The net photosynthetic rates of Jigu 168 and Jinmiao K1 were significantly higher than that of other varieties(P<0.05). Under water deficit treatment,Zhangzagu No. 13 had the best net photosynthetic rate at 33.18 μmol/(m2·s),which was 1.99% lower than that of pre-seedling supplementary irrigation. The transpiration rates of Zhangzagu No. 13 and Jinmiao K1 were better and significantly higher than that of other varieties(P<0.05),4.39% and 3.79% higher under pre-seedling supplemental irrigation treatment than water deficit treatment,respectively. Zhangzagu No. 13 and Jinmiao K1 had better water use efficiency under both treatments. Water deficit led to an increase in leaf photosynthetic water use efficiency,the two varieties increased by 11% and 7%,respectively.【Conclusion】Zhangzagu No. 13 and Jinmiao K1 could be promoted as the dominant varieties of millet for drought resistance and seedling protection in the dry farming area of the Loess Plateau in Inner Mongolia.

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Effects of different treatments on the bacterial community composition and diversity in cucumber continuous cropping soil
JIANG Wei, XUE Guoping, BAI Hongmei, DU Jinwei, ZHU Chunxia, LI Jie, SONG Qingcheng, JI Shuli, WANG Lisheng
Journal of Northern Agriculture    2022, 50 (2): 28-37.   DOI: 10.12190/j.issn.2096-1197.2022.02.04
Abstract1223)      PDF(pc) (2954KB)(33)       Save
【Objective】The impacts of various treatments on the structure and diversity of soil microbial communities on continuous cucumber cropping were investigated to select appropriate soil improvement measures.【Methods】The soil that had been continuously cropped cucumbers for 12 years was treated with straw returning,wheat filling,anti-continuous cropping biological fertilizer,and lime nitrogen disinfection. The Illumina MiSeq sequencing platform was used to conduct a 16S rRNA high-throughput sequencing of soil microorganisms.【Results】 Compared with the control,Chao1 index,Simpson index of the four treatments all increased,with straw returning and anti-continuous cropping biological fertilizer treatment increased significantly( P<0.05). At the bacterial phylum level,different treatments had no effect on the community composition. However the relative abundance of Bacteroidetes increased significantly( P<0.05)in the treatments of straw returning,wheat filling,and anti-continuous cropping biological fertilizer. In the treatment of wheat filling and lime nitrogen disinfection,the relative abundance of Actinobacteria and Gemmatimonadetes increased significantly( P<0.05). In the treatment of anti-continuous cropping biological fertilizer and lime nitrogen disinfection,the relative abundance of Acidobacteria reduced significantly( P<0.05). At genus level in the four treatment,the relative abundance of dominant bacteria Bifidobacterium was significantly reduced( P<0.05)with the appearence Thermopolyspora and Thermomonospora. In wheat filling,the relative abundance of the dominant bacteria Steroidobacteria increased significantly( P<0.05). In straw returning and anti-continuous cropping biological fertilizer,the relative abundance of dominant bacteria chryseolinea, cellvibrio and flavisobacter increased significantly( P<0.05).【Conclusion】In continuous cucumber cultivation,the four improvement measures affected the composition,richness,and diversity of the soil bacterial community. The optimum treatment was adding anti-continuous cropping biological fertilizer,followed by straw returning.
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Analysis on the spatio-temporal evolution of the main food crops planting structure in the three northeastern provinces
YAN Mengchuan, WEI Donglan, WU Yunxia
Journal of Northern Agriculture    2020, 48 (6): 114-118.   DOI: 10.12190/j.issn.2096-1197.2020.06.19
Abstract1217)      PDF(pc) (475KB)(182)       Save
【Objective】To explore the spatio-temporal evolution of the main food crops planting structure in the three northeastern provinces.【Methods】Using the decision tree method,based on MOD13Q1 data,combined with crops phenology data and land use data in the three northeastern provinces,the landscape index was used to analyze the spatio-temporal evolution of the planting structure of the main food crops in the three northeastern provinces—maize,rice and soybean.【Results】From 2000 to 2015,the average patch area of maize and soybean in the three northeastern provinces continued to increase,the patch density continued to decrease,and the patch aggregation degree increased on the whole. From 2000 to 2010,the average patch area of rice continued to increase and the patch density continued to decrease. From 2010 to 2015,the average patch area of rice decreased and the patch density increased. From 2000 to 2015,the patch aggregation degree of rice has been in a stable state.【Conclusion】From the perspective of planting structure,soybean was the most important crop in the three northeastern provinces in 2000,and maize was the most important crop in the three northeastern provinces from 2005 to 2015. From the perspective of planting pattern,the planting of maize and soybean in the three northeastern provinces has been relatively concentrated,and the rice planting showed a slight fragmentation phenomenon from 2010 to 2015,but it was relatively concentrated overall.
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Research progress on the characteristics and development and utilization of Cyperus esculentus
CAO Ziqi, REN Yongfeng, LU Zhanyuan, ZHAO Xiaoqing
Journal of Northern Agriculture    2022, 50 (1): 66-74.   DOI: 10.12190/j.issn.2096-1197.2022.01.09
Abstract1210)      PDF(pc) (1073KB)(120)       Save
Cyperus esculentus is a multi-purpose commercial crop that incorporates grain and oil,feed,food,medicine,and greening,as well as an ecological crop that prevents windstorms and fastens sand,enhances soil fertility,and improves marginal land use efficiency. As a result of its favorable environmental and economic effects,it has been well developed. This paper discussed the characteristics,planting and yield,main nutrient composition,development and utilization of Cyperus esculentus,as well as the challenges that have afflicted its development and the prospects for its industrial development.
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Study on the mechanism of salicylic acid inducing rice blast resistance
FU Jia, LIU Tingting, ZHANG Hui, JIANG Shanqiang, ZHOU Li, MA Dan, TIAN Xuejun, ZHU Deyan, XU Yan
Journal of Northern Agriculture    2022, 50 (4): 47-54.   DOI: 10.12190/j.issn.2096-1197.2022.04.07
Abstract1209)   HTML3)    PDF(pc) (843KB)(61)       Save

【Objective】To clarify the molecular mechanism of salicylic acid induced rice blast resistance.【Methods】Using Hwayoung rice seedlings as test material,the in vitro inhibitory effect of salicylic acid on Magnaporthe oryzae was evaluated. Rice seedlings were induced by salicylic acid(sterile water as control)and then inoculated with Magnaporthe oryzae JC2. The changes in histone acetylation and gene expression related to rice disease course were studied.【Results】Salicylic acid had no significant inhibitory effect on the in vitro growth rate,spore quantity,and spore germination rate of Magnaporthe oryzae. The histone modification levels of H3K9ac and H4K5ac showed an increased trend when the rice seedlings were induced by different concentrations of salicylic acid,and the resistance to rice blast was also improved. Compared with the control plants,in 0.05 mmol/L salicylic acid induced and inoculated 2 d plants(sampled 24 h after inoculation),the expression levels of OsPR1bOsPALOsNH1 and WRKY13 increased extreme significantly(P<0.01).【Conclusion】After rice plants were induced by salicylic acid,the histone acetylation modification level was elevated and the resistance to rice blast was improved.

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Effects of tillage methods on Inner Mongolia dry farming regions soil moisture,temperature and maize yield
CHENG Zhipeng, ZHANG Chengze, WANG Fugui, WANG Zhen, ZHANG Yuezhong, YAN Liwei, LIANG Hongwei, YANG Zhihong, GAO Julin, WANG Zhigang
Journal of Northern Agriculture    2023, 51 (2): 22-32.   DOI: 10.12190/j.issn.2096-1197.2023.02.03
Abstract1204)      PDF(pc) (1614KB)(23)       Save
【Objective】Investigate the effects of different tillage methods on soil moisture,heat dynamics as well as maize dry matter accumulation and yield,to provide theoretical support for maize production in dry farming regions of Inner Mongolia.【Methods】Using random block design,four tillage methods were set up,including conventional ridge cropping(CP),straw off-field no-tillage(NT),straw mulching no-tillage(RNT),and straw mulching strip tillage(RST). Throughout the maize growth stages in 2021—2022,the soil temperature,soil moisture content and maize dry matter accumulation were monitored and measured. Yield was determined at harvest.【Results】Compared with CP,NT,RNT and RST reduced the average soil temperature and 0-5 cm soil active accumulated temperature in the maize growth stages. The 0-5 cm average soil active accumulated temperature of NT and RNT significantly decreased by 69.81 and 127.52 ℃( P<0.05),respectively in the two years,while RST only decreased by 18.94 ℃ with no significant difference( P>0.05). RNT and RST significantly increased soil water content during the maize growth period,with RNT increasing 107.75 and 74.14 mm and RST increasing 71.50 and 37.89 mm compared to CP and NT,respectively. RST increased maize emergence by an average of 1.58%,9.28% and 9.11% compared to NT,CP and RNT respectively. Both dry matter accumulation and yield at maize maturity stage were RST>NT>CP>RNT in the two years. In comparison to CP,NT and RNT,the dry matter accumulation in RST increasing by 11.68%,4.47% and 12.69%,yield increased by 6.06%,4.22% and 7.21%,and water use efficiency(WUE) increased by 7.63%,5.61% and 6.52%,respectively.【Conclusion】Straw mulching strip tillage had better effect in temperature increasing and soil moisture preserving,significantly increased maize dry matter accumulation and yield,was one of the tillage methods to increase maize yield and efficiency in dry farming regions of Inner Mongolia.
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Analysis of yield gap of wheat following rice in Anhui Province
ZHAO Li, HE Xianfang, DENG Cheng, WANG Jianlai
Journal of Northern Agriculture    2023, 51 (3): 1-11.   DOI: 10.12190/j.issn.2096-1197.2023.03.01
Abstract1057)      PDF(pc) (1105KB)(142)       Save
【Objective】To quantitatively analyze the yield gap of wheat following rice in Anhui Province and clarify the space for increasing yield and improving utilization efficiency of resource of wheat following rice.【Methods】Four cultivation patterns:super high yield(SH),high yield and high efficiency(HH),farmer pattern(FP)and inherent soil productivity(ISP)and three difference levels:first-level yield gap(FP-ISP),second-level yield gap(HH-FP),and third-level yield gap(SH-HH) were set up in wheat following rice areas along the Huai River,between Yangtze River and Huai River,and along the Yangtze River in Anhui Province from 2017 to 2020. The dry matter accumulation and leaf area index of over-wintering stage,jointing stage,anthesis stage,filling stage and maturity stage were determined and the effective ear number,kernels per ear,1 000-grain weight and economic coefficient,as well as yield were measured at maturity stage.【Results】Along the Huai River,between Yangtze River and Huai River,and along the Yangtze River in Anhui Province,the first-level yield gap(FP-ISP) of wheat following rice was the largest,with an average of 3 009.6 kg/hm 2. The second-level yield gap and the third-level yield gap were 768.5 and 758.0 kg/hm 2,respectively. Yield gaps varied among different wheat following rice planting regions,with the first-level yield gap decreased gradually from north to south,the second-level yield gap increased slightly from north to south,and the third-level yield gap in the north slightly higher than that of the south. The yield gap of the different levels varied greatly among different years. The effective measures to narrow the first-level yield gap were to increase the effective ear number,kernels per ear,biological yield,economic coefficient and leaf area index in jointing stage.The effective measures to narrow the second-level yield gap were the enhancement of kernels per ear,biological yield and dry matter in anthesis stage. The effective measures to narrow the third-level yield gap were to increase the effective ear number and economic coefficient. Sink number,sink volume,leaf area index in over-wintering stage and jointing stage and dry matter accumulation after flowering were extremely significantly positive correlated( P<0.01) with the yield of wheat following rice. Nitrogen agronomic utilization efficiency,light and accumulative temperature utilization efficiency increased with the improvement of yield.【Conclusion】Increasing grain sink number,expanding sink capacity and improving grain sink quality were the effective means to narrow the yield gap and increase yield of wheat following rice in Anhui Province.
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Research on the construction of sugar beet BvCENH3 gene mutants based on CRISPR/Cas9 technology
HAN Ping′an, TANG Kuan′gang, CHANG Yue, SUN Ruifen, WANG Liang, ZHANG Ziqiang, FU Zengjuan, ZHAO Shangmin, WU Xinrong, LI Xiaodong
Journal of Northern Agriculture    2023, 51 (2): 1-11.   DOI: 10.12190/j.issn.2096-1197.2023.02.01
Abstract1024)      PDF(pc) (1023KB)(138)       Save
【Objective】The CRISPR/Cas9 technology was used to edit the BvCENH3 gene in sugar beet aiming to establish an efficient genome editing system.【Methods】Taking sugar beet BvCENH3 gene as the editing target,double candidate targets were selected to construct gene editing vector. Transgenic sugar beet plants were produced through agrobacterium-mediated transformation. Next generation sequencing technology was utilized to identify the mutation types and droplet digital PCR was employed to screen low-copy mutant plants.【Results】82 transgenic sugar beet plants were obtained,40 of which were successfully edited,with an editing efficiency of 48.78%. Target 1 was more efficient than target 2. There were five mutation types,including single base substitution(T→G、A→C) and base deletion(TC、TCTC deletion). 23 low-copy edited plants were selected,with BvCENH3 insertion copy numbers ranging from 1.1 to 1.9.【Conclusion】Successfully edited BvCENH3 in sugar beet with 40 BvCENH3 gene mutants obtained. Preliminary established sugar beet genome editing system,laying theoretical and technical foundations for sugar beet haploid breeding.
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Journal of Northern Agriculture    1989, (4): 18-18.  
Abstract1013)      PDF(pc) (87KB)(10)       Save
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Analysis of agricultural irrigation status and water saving potential in Hetao Irrigation Area——A case study in Hanggin Rear Banner in Inner Mongolia
CHEN Guangfeng, LIU Yujie, YAN Dong, BAI Yongxing, LI Bei, LIU Xiaoyan, BAI Yunlong, WU Yong, GAO Xiangzhao, DU Sen
Journal of Northern Agriculture    2020, 48 (2): 65-69.   DOI: 10.12190/j.issn.2096-1197.2020.02.10
Abstract992)      PDF(pc) (1162KB)(668)       Save
【Objective】To reveal the current situation and water-saving potential of main crops irrigation in Hetao Irrigated Area and put forward optimization suggestions.【Methods】The irrigation situation was analyzed by using the survey data and literature data of 310 households in Hanggin Rear Banner.【Results】In this region, wheat, corn and sunflower were irrigated for many times with high irrigation quota, and the average crop irrigation water productivity was only 1.43 kg/m 3.By adopting water-saving planting technology and scientific irrigated in Autumn, the three main crops could save 200 million-315 million m 3 water per year.【Conclusion】Since the irrigation behavior and low water resource utilization efficiency in Hetao irrigated area, large water-saving space can be ture by combining the border irrigation and high efficient water-saving irrigation.
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Comprehensive evaluation of agronomic,quality traits and adaptability of new naked oat varieties (lines)
ZHENG Chengzhong, XU Zhenpeng, ZHANG Zizhen, WANG Qianjun, MEI Xue, SUN Ying, WANG Fengwu, YE Lu
Journal of Northern Agriculture    2023, 51 (2): 12-21.   DOI: 10.12190/j.issn.2096-1197.2023.02.02
Abstract986)      PDF(pc) (981KB)(59)       Save
【Objective】To screen naked oat varieties(lines) suitable for cultivation in Ulanqab City,Inner Mongolia and lay a theoretical foundation for the introduction of oat varieties,breeding,demonstration and promotion of new varieties in the area.【Methods】Using 12 newly bred naked oat varieties(lines) in China as experimental material,in single factor randomized block experiments,the growth period,main agronomic traits,yield and quality traits of the different oat varieties(lines) were analyzed.【Results】All of the 12 naked oat varieties(lines) tested could mature normally in the local area. The varieties(lines) with better main agronomic traits were:201229-1-2,Zhangyou No.8,Bayou No.18. Bayou No.18 had better performance in plant height(136.10 cm),main panicle length(22.03 cm),grain weight per spike(2.94 g),tiller number(3.58) and 1000-grain weight(30.14 g). 201229-1-2 performed better in tiller number(4.20),spikelet number(35.73) and grain weight per spike(2.06 g). Zhangyou No.8 had better performance in 1 000-grain weight(30.14 g). The varieties(lines)with better yield performance were Bayou No.18,201229-1-2 and Baiyan No.16,at 3 250.05,3 330.00 and 3 157.80 kg/hm 2,respectively. The varieties(lines) with better quality traits were Zhangyou No.9(highest protein content at 16.57%),Jinyan No.18(highest β-glucan content at 5.00%),Weiduyou No.5(highest starch content at 61.98%),and 201229-1-2(highest crude fat content and grain bulk density at 5.51% and 709.00 g/L). Principal component analysis showed that the comprehensive scores of Bayou No.18,Baiyan No.16,Zhangyou No.8,and 201229-1-2 were higher,with relatively prominent yield traits and nutritional quality traits.【Conclusion】The naked oat varieties(lines) with better comprehensive traits were Bayou No. 18,Baiyan No. 16,Zhangyou No. 8,and 201229-1-2. They were suitable for promotion and cultivation in Ulanqab City,Inner Mongolia,with Bayou No. 18 being particularly prominent.
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Analysis of Huang-Huai-Hai maize heterotic pattern based on SSR molecular marker technology
CHAI Wenbo, LI Shufen, LI Hongtao, XU Hanyuan, ZHU Qing, WANG Jun
Journal of Northern Agriculture    2022, 50 (6): 17-24.   DOI: 10.12190/j.issn.2096-1197.2022.06.03
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【Objective】Analyzing the heterotic pattern of superior maize breeding lines L239 and L7221 to reveal the genetic resources of superior varieties and to provide reference for maize breeding in the Huang-Huai-Hai region. 【Methods】The genetic diversity of L239 and L7221 in 42 maize inbred lines was analyzed by SSR molecular marker technology to determine the heterosis pattern of maize in Huang-Huai-Hai region.【Results】Analysis of 42 maize inbred lines showed that the allele number of the 56 primers was 2-9,and the polymorphism information content was 0.43-0.86. The genetic distance between L239 and CA375 was shortest at 0.50. The genetic distance between L7221,LX9801 and Nongda 178 were shorter at 0.43 and 0.56,respectively. According to the genetic similarity analysis of 42 maize inbred lines,L7221 mainly belonged to Tangsipingtou group,and L239 mainly had closer relationship with the germplasm resources of the improved Reid group.【Conclusion】L7221 was closer to many inbred lines of Tangsipingtou group,and L239 was closer to many inbred lines of improved Reid group. The L239 and L7221 heterosis pattern was improved Reid group×Tangsipingtou group.

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Physiological responses and saline-alkali tolerant evaluation of 15 sugar beet varieties to saline-alkali stress
HUANG Chunyan, SU Wenbin, GUO Xiaoxia, LI Zhi, JIAN Caiyuan, TIAN Lu, FAN Fuyi, REN Xiaoyun, GONG Qianheng, ZHANG Qiang
Journal of Northern Agriculture    2020, 48 (4): 1-9.   DOI: 10.12190/j.issn.2096-1197.2020.04.01
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【Objective】 In order to reveal the physiological response of different sugar beet varieties to saline-alkali stress and screen salt-alkali tolerant varieties.【Methods】 15 main sugar beet varieties were used as materials to simulate the effects of different degrees of salt-alkali stress(control,light,moderate and severe saline-alkali soil)on the growth and physiological indexes of sugar beet seedlings under natural soil conditions.【Results】 Compared with the control,the seedling emergence rate,fresh weight,dry weight and chlorophyll content of 15 sugar beet varieties decreased in varying degrees with the increase of salt-alkali stress.The five varieties of seedling emergence rate decreasing slightly: were MA3001,MA2070,KWS1176,KWS9149,MA079,and the five varieties of fresh weight decreasing slightly: were MA3001,KWS1176,KWS2323,NEI2499,BETA379,and the five varieties of dry weight decreasing slightly:were KWS1176,BETA5043,MA3001,MA079,BETA379.The decreases of MA3001 and KWS1176 were smaller so that these were the salt-alkali tolerant varieties.The plasmalemma permeability and MDA content in leaves of 15 sugar beet varieties increased with the increase of salt-alkali stress,and small the increase ofor the salt-alkali tolerant sugar beet seedlings was smaller.With the increase of salt-alkali stress,the SOD activity of 15 sugar beet varieties was different,and the POD activity of different varieties showed a single peak curve change,and SOD activity and POD activity of salt-alkali tolerant varieties decreased slightly in severe saline-alkali soil.【Conclusion】 MA3001 and KWS1176 are salt-alkali tolerant sugar beet varieties.They can effectively reduce the membrane lipid peroxidation and the plasmalemma permeability of leaves by strengthening the activity of antioxidant enzymes,thereby improving the salt-alkali tolerance of sugar beet seedlings.
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Effects of different fertilization treatments on oat yield,nutrient absorption and water and fertilizer utilization efficiency
ZHANG Jun, DUAN Yu, LIU Yahong, LIANG Junmei, WANG Bo, AN Hao, LIAN Haifei
Journal of Northern Agriculture    2020, 48 (5): 62-68.   DOI: 10.12190/j.issn.2096-1197.2020.05.11
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【Objective】Explore the fertilization effect of oats in the northern Yinshan Mountain,and provide scientific basis for the rational fertilization of dryland oats in the northern Yinshan Mountain.【Methods】Based on the long-term positioning experiment(started in 2004)of Wuchuan dry farming experimental station in Inner Mongolia,the effects of different fertilization measures on oat yield,plant nutrient absorption and oat water and fertilizer utilization efficiency under long-term fertilization conditions were studied.【Results】Oat yield,1 000-grain weight,plant high,panicle length and spikelet number per plant could be significantly increased under the combination use of chemical and organic fertilizer(NPK+O) and balanced fertilization(NPK).The grain yield under NPK+O and NPK conditions could be increased by 49.86% and 38.97% compared with oat planted without fertilization(CK).The oat LAI,biomass,and amount of absorption of N,P,and K could be significantly increased under NPK+O and NPK treatments,the maximum LAI,and biomass were 2.62 and 6 066 kg/hm 2,respectively.The max amount absorption of N,P and K was 115.28,26.77 and 132.92 kg/hm 2,respectively.Combined application of NPK+O and NPK could significantly increase the water use efficiency in oats,reaching 8.17 and 7.84 kg/(hm 2·mm),respectively,which were 46.5% and 40.0% higher than CK.The use efficiency of N,P 2O 5,and K 2O under NPK was 36.37%,17.93%,and 59.92%,respectively.Each 100 kg oat absorbed N 3.38-6.21 kg,P 2O 5 1.09-1.49 kg,K 2O 3.98-7.43 kg,with a value of 4.93,1.31,and 5.77 kg,respectively.【Conclusion】The combination use of chemical and organic fertilizer(NPK+O)and balanced fertilization(NPK)could significantly increase yield,nutrient absorbs,water and fertilizer utilization efficiency of oats,which were nutrient management models for high efficient resource utilization and oat yield increase.
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Study on the suitable planting density and high-yield cultivated varieties of maize in Hetao irrigation district
LIU Siqi, HE Zhongping, BAO Junwei, FAN Xiurong, GAO Haiyan, ZHANG Yan, ZHANG Hongping, DAI Cheng, SUN Xiuyun, ZHAO Xiaoyu
Journal of Northern Agriculture    2024, 52 (3): 29-37.   DOI: 10.12190/j.issn.2096-1197.2024.03.03
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【Objective】To screen the suitable maize planting density and varieties in Hetao irrigation district of Inner Mongolia,and provide reference for the improvement of maize yield per unit area based on this.【Methods】The experiment was carried out in Bayannur City,Inner Mongolia from 2022 to 2023. A two-factor randomized block design was adopted,and the main treatment was plant density(D1:90 000 plants/hm 2,D2:97 500 plants/hm 2,D3:105 000 plants/hm 2,D4:112 500 plants/hm 2,D5:120 000 plants/hm 2),and varieties were sub-treated(Sitai 112,Lilong 170,Mingyu 268,Jinyuan 118). The effects of different densities on agronomic traits,yield components and yield of each maize variety were analyzed,and their economic benefits were calculated.【Results】At 5 densities,the reproductive growth of Sitai 112 was 3 days earlier than that of other varieties. At D3 density,Mingyu 268 plant height and ear height both were the highest,which were 3.18 and 1.33 cm,respectively. At D5 density,the lodging rate and hollow bar ratio of Mingyu 268 both were the highest,which were 30.40% and 14.21%,respectively. With the increase of density,the ear length of each variety gradually decreased and the values of Sitai 112,Lilong 170,Mingyu 268 and Jinyuan 118 reached the maximum at the density of D1,which were 20.10,21.55,18.75 and 20.85 cm,and there was no significant difference in ear diameter among varieties( P>0.05). At the density of D1 and D2,the yield and net income of Sitai 112 were highest,which were 14.68,15.01 t/hm 2 and 27 394,28 017 yuan/hm 2. At the density of D3 and D4,the yield and net income of Lilong 170 were highest,which were 17.09,17.00 t/hm 2 and 32 504,32 206 yuan/hm 2. At the density of D5,the yield and net income of Jinyuan 118 were highest,which were 13.13 t/hm 2 and 23 585 yuan/hm 2.【Conclusion】In Hetao irrigation district of Inner Mongolia,the high-yield cultivated varieties were Sitai 112 at 90 000 and 97 500 plants/hm 2,Lilong 170 at 105 000 and 112 500 plants/hm 2,and Jinyuan 118 at 120 000 plants/hm 2.
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Anatomical characteristic of vegetative organs and cuttings rooting of Portulaca oleracea L.
HU Shun, WANG Yong, LIU Huijuan, HAN Fengying, WU Yunxia, MU Zongjie, WANG Yong
Journal of Northern Agriculture    2020, 48 (4): 83-89.   DOI: 10.12190/j.issn.2096-1197.2020.04.13
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【Objective】 To determine the ecological adaptive capacity and rooting type of Portulaca oleracea L. for its better develop and utilize.【Methods】 The anatomical vegetative organs and cuttings rooting were observed by paraffin section.【Results】 The roots and stems of purslane were stout and contained more parenchyma.The root had secondary structures such as pericarp and secondary vascular tissue,and xylem was well developed.There were scattered vascular bundles in the stem,and cortex and marrow were more developed.The leaves were typical heterofacial,with mucinous cells,and the main veins were arranged with two external tough vascular bundles.There were annular fiber bundles outside petiole and main vascular bundle.Crystals were found in parenchyma cells of roots,stems and leaves.Adventitious roots of stem cuttings originated from parenchyma cells near the interfascicular cambium or phloem.【Conclusion】 The vegetative organs of Portulaca oleracea L. had the characteristics of resistance to dry and salt.The root of stem cuttings was the original type of inducing root and easy to take roots.
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Root-knot nematode control effect of 41.7% Lufuda SC different application methods in tomato
XI Xianmei, HUO Hongli, HAN Ping′an, HUANG Shuo, RONG Xiaojun, GE Tong, CAO Chunling
Journal of Northern Agriculture    2021, 49 (6): 98-102.   DOI: 10.12190/j.issn.2096-1197.2021.06.13
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【Objective】To determine the control effect of 41.7% Lufuda SC different application methods on root-knot nematode of tomatoes.【Methods】41.7% Lufuda SC at a dosage of 0.030 mL/plant was used to irrigate the root,spray on ridge surface,and broadcast on strip surface. 20% fosthiazate FW for root irrigation,10% fosthiazate GR at 30 kg/hm 2 soil spraying and water were used as controls. The effect of different treatments on root-knot nematode control was recorded.【Results】The population declining rates of root-knot nematode by 41.7% Lufuda SC treatments to irrigate the root,spraying on ridge surface and broadcasting on strip surface were 46.39%,23.21%,and 22.95% respectively. The control effects were 64.19%,59.74% and 50.22% respectively. The population declining rates for 20% fosthiazate FW and 10% fosthiazate GR were 42.67% and 29.89%,the control effects were 62.21% and 48.46% respectively. 41.7% Lufuda SC on root irrigation enhanced yield by 27.07% compared to water treatment.【Conclusion】For 41.7% Lufada SC,irrigation on roots method had much better effect in tomato root-knot nematode control than spraying on ridge surface,broadcasting on strip suface and all controls,increased yield significantly as well.
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Research progress of the straw returning on soil improvement in saline-alkali land
ZHANG Xiumin, GAO Riping, KANG Wenqin, WANG Weini, PAN Zuntian, HUANG Jie, GAO Shanming, YU Xiaofang, JING Yupeng
Journal of Northern Agriculture    2021, 49 (5): 85-92.   DOI: 10.12190/j.issn.2096-1197.2021.05.12
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Saline-alkali land is an important reserve cultivated land resource in China. The development and utilization of saline-alkali cultivated land has important practical significance for meeting population and food needs of as well as agriculture long-term sustainablility. Straw returning can improve soil structure and fertility,improve the quality of cultivated land,and reduce the salt accumulation in topsoil,making it an effective measure for improving saline-alkali soil. This paper summarized the current state of domestic straw returning technology,explained the principle and application of straw returning technology to improve saline-alkali soil,and discussed the effects of improvement on saline-alkali soil from the aspects of soil physical properties,chemical properties,biological characteristics and crop yield. The impact of improved saline-alkali straw returning technology application foreground was prospected.
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Applied research progress on agricultural plant enzyme nutrition solution
PANG Minhui, ZUO Qiang, SONG Daping, GAO Lijuan, LIU Dongsheng, ZOU Guoyuan
Journal of Northern Agriculture    2019, 47 (5): 60-64.   DOI: 10.3969/j.issn.2096-1197.2019.05.11
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Plant enzyme nutrition solution is an enzyme preparation that is generally made from natural plant tissues or crop residues such as leaves,flowers,and fruits,which are mixed with brown sugar and water,and then fermented for several months.It contains plentiful probiotics,nutrient elements,natural plant hormones,and organic acids,which can build a good soil micro-ecological environment and have an important ecological combined effect that can promote plant growth and so on. The research status of the plant enzyme nutrition solution on the preparation process,composition and applications was introduced,the prospect was envisioned,and the suggestions were also put forward.
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Journal of Northern Agriculture    2005, (B12): 368-368.  
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Effects of different NPK fertilizer application ratio on biomass of Brassica chinensis L.
CAI Wuning, LI Xiaoyan, YUAN Qi, ZHU Kun
Journal of Northern Agriculture    2020, 48 (1): 51-55.   DOI: 10.12190/j.issn.2096-1197.2020.01.10
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【Objective】In order to study the optimal NPK ratio for Brassica chinensis L..【Methods】Part of “3414” fertilizer effect strategy was used to design Brassica chinensis L. fertilization experiment. Combined with the changes of soil nutrient composition,the biomass and soil nutrient content of Brassica chinensis L. were compared under different treatments. 【Results】The fresh weight and NPK content of Brassica chinensis L. Using N 2P 2K 2 fertilization strategy were significantly higher than other treatments. This strategy was highly beneficial to maintain the soil nutrition structure and prevent soil alkalization. It was determined that the N 2P 2K 2 fertilization strategy was better.【Conclusion】 On this basis,the optimal fertilization strategy for Brassica chinensis L. was revised to be 2.373∶2∶2 by curve fitting of nitrogen fertilizer effect on the P 2K 2 level. Using the optimal NPK ratio,the fresh weight and NPK content of Brassica chinensis L. were close to the maximum point,making the return the highest.
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Research advances on medicinal and edible value of sowthistles
HU Shun, WANG Yong, WANG Yong, HAN Fengying, LIU Huijuan, PANG Jie, MU Zongjie
Journal of Northern Agriculture    2019, 47 (5): 90-95.   DOI: 10.3969/j.issn.2096-1197.2019.05.17
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Sowthistles are formed by a class of functional plants with medicinal and edible value.Bitter cabbage is namedbecause of bitter,and wild resources are very rich.In this paper,the research status were summarized including the nutrition ingredient,gathering food,artificial cultivation,tissue culture,germination characteristics,karyotype analysis,bacteriostasis,active components,pharmacological and clinical application,development and utilization of sowthistles,and the future research and the development direction were envisioned.
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Inner Mongolia distributed national key protected wild plants and the floristic characteristics
LIU Bo, LIU Guanzhi, LIU Guohou, JIN Rong, CHENG Xinghua
Journal of Northern Agriculture    2022, 50 (3): 44-52.   DOI: 10.12190/j.issn.2096-1197.2022.03.06
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【Objective】According to the adjusted List of National Key Protected Wild Plants,the species distributed in Inner Mongolia were statistically sorted out to form a list of national key protected wild plants distributed in Inner Mongolia,providing guidance and services for the protection of wild plants in Inner Mongolia.【Methods】According to the List of National Key Protected Wild Plants approved and released by the State Council on August 7,2021,the list of national key protected wild plants in Inner Mongolia was organized in accordance with the region′s current plant distribution. The floristic geographical components were studied using floristic research techniques.【Results】A list of national key protected wild plants distributed in Inner Mongolia was formed,including 47 species of national key protected wild plants,belonging to 26 families and 37 genera. Among them,there were 1 species of algae,2 species of fungi,1 species of bryophytes,1 species of gymnosperms,and 42 species of angiosperms. Nostoc flagelliforme Born. et Flah. and Aldrovanda vesiculosa L. were the only two species of wild plants under national first-class protection;the others were under national secondary protection. The families with the largest number of species in angiosperms were Poaceae and Orchidaceae,both of which were 7 species. And the genera with the largest number of species were Cypripedium L. and Elymus L.,both of which were 5 species. The life forms of spermatophyte were mainly perennial herbs,with a total of 28 species. And the ecotype was dominated by mesophytes,with a total of 23 species. The floristic geographical distribution of species included 8 types and 5 sub-types,most of which were North East Asian distribution species,gobi distribution species and pan-Arctic distribution species. There were 3 endemic or nearly endemic genus in Inner Mongolia,including Tetraena Maxim., Potaninia Maxim. and Tugarinovia Iljin,and 11 endemic or nearly endemic species in Mongolian plateau.【Conclusion】The newly formed list of national key protected wild plants distributed in Inner Mongolia reflected the conservation status of wild plant in Inner Mongolia,as well as the background and characteristics of Inner Mongolia flora.
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Effects of mixed application of microbial fertilizer and chicken manure on apple seedling growth and saline-alkali soil improvement
MIAO Ping, LI Fakang, CUI Guodong, WANG Zhenyu
Journal of Northern Agriculture    2022, 50 (5): 41-47.   DOI: 10.12190/j.issn.2096-1197.2022.05.06
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【Objective】The effects of microbial fertilizer mixed with chicken manure on the growth of apple seedlings and the improvement of saline-alkali soil in apple field were studied in order to provide theoretical basis for the improvement of saline-alkali soil in apple field in Gansu Province.【Methods】Five treatments were set up:no fertilizer(blank,T0),single microbial fertilizer(control,T1),50 g/(kg soil) chicken manure+microbial fertilizer(T2),75 g/(kg soil) chicken manure+microbial fertilizer(T3),100 g/(kg soil) chicken manure+microbial fertilizer(T4). At 30,60,90,and 120 d,the effects of various treatments on apple seedling development,root activity and soil pH value,total salt content,bulk density,enzyme activities were studied.【Results】Combined application of chicken manure and microbial fertilizer,the leaf area of apple seedling leaves had no significant change(P>0.05). At 120 d,in T4 treatment,the leaf SPAD value of apple seedling was the largest,15.75% and 12.88% higher than that in T0 and T1 treatment,respectively. The soil total salt content,pH value,and bulk density were the lowest, 15.51%,6.53%,and 23.82% lower than T0 treatment,and 14.20%,3.49%,and 20.34% lower than T1 treatment,respectively. At 120 d,in T3 treatment,apple seedling had the highest root activity,12.15% and 4.41% higher than those in the T0 and T1 treatments,respectively. The ALP activity,S-UE activity and NAG activity of saline-alkali soil in apple field were the highest,increased by 65.52%,29.96% and 116.38% respectively compared with T0 treatment and 17.07%,14.95% and 34.23% respectively compared with T1 treatment.【Conclusion】The growth of apple seedlings and saline-alkali soil were improved by the treatments of 75 g/(kg soil) chicken manure+microbial fertilizer and 100 g/(kg soil)chicken manure+microbial fertilizer. The longer the treatment period,the more pronounced the effects on apple seedling growth and soil improvement were.

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Evaluation of sensory qualities of different tomato varieties
ZHAO Yan, QIU Pengcheng, WU Lingbo, WANG Le, LIU Jinglei, TAO Sarula
Journal of Northern Agriculture    2022, 50 (5): 87-95.   DOI: 10.12190/j.issn.2096-1197.2022.05.13
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【Objective】To screen fresh tomato varieties suitable for planting in Ordos,Inner Mongolia,and to provide reference for the establishment of a sensory quality evaluation system for tomato fruit.【Methods】The five sensory quality indices of the 19 fresh tomato varieties(10 cherry tomato varieties and 9 large tomato varieties)introduced to Ordos in 2022 were evaluated using the questionnaire survey combined with comprehensive evaluation and fuzzy comprehensive evaluation. A questionnaire survey on the taste of tomatoes was conducted at the same time to confirm the rationality of the evaluation index and weight.【Results】The sensory quality evaluation scores of 10 cherry tomato varieties were ranked from high to low as Fengzhu,Jinbeibei,Fushan 88,Kiwi tomato,Mizhu No. 3,Qianxi,Hanghuangying No. 1,Hangcaiying No. 1,Hangfenying No. 6,Zicaixia tomato. The sensory quality evaluation scores of 9 large tomato varieties were ranked from high to low as Hangfengaotang,Xiangfei No. 9,Agatha 505,Provence,Milumifen,Xiutailang,Youshi No. 3,Tianmi 199,Antares. The reason for desirable flavor was “moderate sweet and sour”. The reason for undesirable taste was “thick skin”.【Conclusion】Cherry tomato Fengzhu,Jinbeibei,Fushan 88,and Kiwi tomato,large tomato Hangfengaotang,Xiangfei No. 9,Agatha 505,and Provence had better sensory quality and were recommended for promotion. The sensory quality evaluation index and weight settings were relatively reasonable.

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Adaptability evaluation of new varieties of sugarbeet with high quality and disease resistance in Ta′e Reclamation Area in Xinjiang
LUO Jingjing, WANG Heya, WANG Kang, WANG Fucheng, WANG Xia, CUI Yu, BAI Ruxiao, YANG Zhe
Journal of Northern Agriculture    2021, 49 (1): 17-22.   DOI: 10.12190/j.issn.2096-1197.2021.01.03
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【Objective】 To screen new varieties of sugarbeet with high quality and disease resistance in Ta′e Reclamation Area in Xinjiang.【Methods】 In the 4-year continuous cropping disease nursery of sugarbeet,a randomized block arrangement design was used to conduct a plot comparison test. Each treatment was repeated 3 times. The local main growing variety Beta796 was used as the control variety. The 9 introduced sugarbeet varieties KWS7748,KWS9968,KWS9899,KWS9962,KWS0066,KWS3410,KWS3354,Beta468,KWS5599 were comprehensively analyzed for their variety adaptability,disease resistance,root yield and sugar yield and other traits.【Results】 The KWS7748 and Beta468 had outstanding performance in disease resistance,root yield,sugar yield and other traits,which were better than the control variety Beta796. The root yield and sugar yield of variety KWS7748 were 43.15% and 43.50% higher than Beta796,and the root yield and sugar yield of Beta468 were 25.83% and 34.47% higher than Beta796,respectively.【Conclusion】 KWS7748,Beta 468 were suitable for cultivation in Ta′e Reclamation Area and could be promoted in the area.
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Research progress of mutation breeding in soybean
JIN Kunpeng, LI Dan, LI Xiaoxia, CAO Jinjun, HAN Wenqing, LIU Yongzhong, LI Wanxing
Journal of Northern Agriculture    2020, 48 (5): 30-33.   DOI: 10.12190/j.issn.2096-1197.2020.05.06
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Mutation breeding is a widely used breeding method,which provides a means for the selection and breeding of new soybean varieties.This paper reviews the application mechanism of chemical mutagenesis(EMS,EI),physical mutagenesis(ionizing radiation,laser)and aerospace mutagenesis breeding in soybean breeding.The effects of mutagenesis on phenotype,physiology,biochemistry,disease resistance of soybean plant and the screening and identification methods of mutants were analyzed,and also the future soybean mutagenesis breeding technology was prospected.
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Scanning electron microscope observation and RVA spectrum characteristics of starch granules in a rice variety with low glutelin content
GUO Tao, ZHANG Huanxia, XUE Fang, WANG Haifeng, JIANG Yanfang, FANG Wenwen, LIN Xiangqing, ZHANG Shiyong
Journal of Northern Agriculture    2020, 48 (5): 49-54.   DOI: 10.12190/j.issn.2096-1197.2020.05.09
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【Objective】To determine the effect of reduced glutenin content on starch grain structure and RVA(rapid viscosity analyzer)spectrum characteristics.【Methods】 Investigate the effect of reduced glutenin content on grain structure and starch RVA spectrum characteristics by the Scanning Electron Microscope(SEM) and viscosity analyzer.【Results】Compared with the background parent Shengdao 735,the plant height of Shengdao LG03 was reduced,the number of grains per panicle was slightly reduced,the total protein content was reduced,and there were no significant differences in other agronomic traits.Compared with Shengdao 735,new low-gluten content Shengdao LG03 had no significant change on the morphology of starch granules,but the size uniformity was reduced,and starch RVA curve showed that peak viscosity(PKV),hot paste viscosity(HPV),cool paste viscosity(CPV),breakdown viscosity(BDV),pasting temperature(PaT)and peak time(PeT)all had downward trend,while consistence viscosity(CSV)and setback viscosity(SBV)increased.【Conclusion】 The decrease of gluten content had little effect on the morphology of rice starch granules,but the uniformity of particle size was reduced,that may be the reason of the changes of starch RVA characteristic values.
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Identification of the pathogen of Hemerocallis citrina rust disease and the rust resistance of different Hemerocallis citrina varieties
ZHANG Lijie, ZHOU Lingling, LIU Shuhua
Journal of Northern Agriculture    2022, 50 (5): 73-81.   DOI: 10.12190/j.issn.2096-1197.2022.05.11
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【Objective】To identify the characteristics of rust pathogens in Hemerocallis citrina and the rust resistance of different Hemerocallis citrina varieties.【Methods】32 Hemerocallis citrina rust samples were collected from Hunan Province,Gansu Province,Shaanxi Province and Jiangsu Province. The uredinium and urediniospores of Hemerocallis citrina were observed and measured under microscope. One of the Hemerocallis citrina rust sample(yx-30)was then selected and the telia and teliospores were observed and measured. The rust resistance of 50 samples of Hemerocallis citrina germplasm resources to strain yx-26 was identified by artificial inoculation.【Results】The uredinium of Hemerocallis citrina rust samples were mostly on the abaxial side of leaves,forming orange or yellow-brown spots with diameters of 0.28-1.96 mm. The urediniospores were almost spherical or oval,yellow and unicellular and 16.0 to 21.5 μm by 17.2 to 32.4 μm in size. The wall thickness was 1 to 3 μm. The telia of Hemerocallis citrina rust sample was dark brown and flocculent. The teliospores were oval,rod-shaped,yellow,bicellular,with constriction in the middle,and shaped like gourd. The size was 68.0 to 81.0 μm by 19.4 to 22.2 μm. The length of sporocyst stalk was 11 to 16 μm. The rust resistance to yx-26 strain of the 50 Hemerocallis citrina germplasm resources were 11 cultivars with high resistance,21 cultivars with moderate resistance,8 cultivars susceptible,and 10 cultivars highly susceptible.【Conclusion】The morphological characteristics of uredinium of Hemerocallis citrina were similar,while the morphological sizes of urediniospores were slightly different. The telia of Hemerocallis citrina rust sample was dark brown and flocculent. The teliospores were yellow,bicellular and shaped like gourd. The rust-resistant varieties accounted for 3/5 of the 50 samples of Hemerocallis citrina germplasm resources,which could provide reference for the planting of rust resistant varieties of Hemerocallis citrina in different regions.

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Application status and prospect of physical mutagenesis technology in medicinal fungi breeding
LIU Chaoxiong, FENG Tingting, LI Yajiao, MU Zongjie, SUN Guoqing, GUO Jiufeng, YU Chuanzong, WANG Haiyan, LIU Li
Journal of Northern Agriculture    2021, 49 (2): 56-62.   DOI: 10.12190/j.issn.2096-1197.2021.02.09
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Medicinal fungi refers to large fungi that are widely used in the field of traditional Chinese medicine and have certain therapeutic effects. Increasing and improving the yield and quality of medicinal fungi is an important goal of medicinal fungi breeding,and compared with other mutagenesis methods,physical mutagenesis has played an important role in the field of medicinal fungi mutagenesis breeding. This article reviews 9 different physical mutagenesis breeding methods including ultraviolet mutagenesis,ionizing radiation mutagenesis,laser mutagenesis,ion beam mutagenesis,ultrasonic mutagenesis,plasma mutagenesis,neutron mutagenesis,space mutagenesis,and compound mutagenesis and the research progress of physical mutagenesis technology in the field of medicinal fungi,and prospects for its application.
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ISSN 2096-1197
CN 15-1375/S
CODEN BNXEAR
chief editor: LU Zhanyuan
Responsible Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Sponsor Unit: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences
Editing Unit: Editorial Department of Journal of Northern Agriculture
Address: Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,22 Zhaojun Road,Hohhot,China
E-mail: bfnyxb@126.com